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Field-Flow Fractionation (FFF)

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Overview

Characterization of large molecules and particles by Field-Flow Fractionation (FFF)
FFF is a family of analytical techniques which are ideally suited for very gentle and high resolution separation and characterization of large molecules and particles, which traditional chromatographic techniques cannot perform.

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Postnova AF2000 MT mid temperature MultiFlow FFF system
The system can be configured for Asymmetric Flow Field-Flow Factionation (AF4), Hollow Fiber Flow Field-Flow Factionation (HF5) and Size Exclusion Chromatography (SEC).

Postnova CF2000 Centrifugal FFF system
The system can separate and distinguish relative size differences of 5% only and can be used to monitor absolute size differences down to 1 nm. These unique features make it the ideal choice for any nanoparticle research and QC tasks.

Postnova TF2000 Thermal FFF system
The system can separate simultaneously polymers and particles without the limitations associated with GPC/SEC. The separation takes place according to molecular weight AND chemical composition, thus making the TF2000 Series the ideal choice for difficult polymer separation.

Postnova 21-angle MALS detector - the ideal detector for ultra-high molar mass
The Postnova 21-angle MALS detector has been especially developed for coupling with FFF systems – to the opposite of previously available MALS detectors which were designed with chromatography (SEC/GPC) in mind, that is for smaller molar mass.

High sensitivity online Dynamic Light Scattering detector, special customized for use connected to FFF
Online DLS measurements using a special flow cell can be perfomed to obtain particle size (Rh) and molecular weight (Mw) distributions of particles and molecules separated by Postnova Asymmetric Flow FFF (AF4), Centrifugal FFF (CF3) and Thermal FFF (TF3) systems.

Model 3310 viscometer detector
The Postnova model 3310 viscometer detector is a state-of-the-art intrinsic viscosity detector for Field-Flow Fractionation (FFF) and Size Exclusion Chromatography (GPC/SEC). It is based on the proven Wheatstone-Bridge design for measuring the intrinsic viscosity of macromolecules in solution. The instrument is designed in the modern and compact Postnova FFF detector design.

Postnova model 8050 FFF fraction collector
Model 8050 employs the latest state-of-the-art robotic sample and fluid transfer technology such as a double needle, special wash routines for minimal carry over and direct fractionation into closed vials. It can be operated with different microwell plates, Eppendorf caps as well as chromatography and preparative vials. It is available as biocompatible and metal-free version.

Events & News

See you at the Nordic Polymer Days 2017 in Stockholm!
P
ostnova and Norlab will be sponsoring the conference and participating in the exhibition, which will be open throughout the meeting. Michel Palu (Norlab, distributor of Postnova in the Nordic countries) will be attenting the NPD. Michel will be pleased to discuss with you about the Postnova SEC/FFF Platform, the ultimate tool for the separation, characterization and fractionation of polymers and nanostructures.

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Literature

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Date of creation: 
1 October 2015
Library code: 
10163
Postnova AF2000 MF supplies and consumables
Date of creation: 
24 July 2017
Library code: 
11114
Date of creation: 
1 October 2015
Library code: 
10164
The system can separate simultaneously polymers and particles without the limitations associated with GPC/SEC
Date of creation: 
1 October 2015
Library code: 
10165
Multi-Angle Light Scattering detector (MALS) model 3621
Date of creation: 
1 October 2015
Library code: 
10220
Dynamic Light Scattering detector model Zetasizer Nano ZS173
Date of creation: 
1 October 2015
Library code: 
10221
Postnova model 3310 online viscometry for FFF and GPC
Date of creation: 
25 July 2017
Library code: 
11122
Postnova model 8050 FFF fraction collector
Date of creation: 
31 January 2016
Library code: 
10639