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Supported Liquid Extraction

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Overview


Sample preparation using Supported Liquid Extraction

The Supported Liquid Extraction (SLE) process is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water immiscible solvent systems for analyte extraction. However, instead of shaking the two immiscible phases together, the aqueous phase is immobilized on an inert diatomaceous earth based support material and the water immiscible organic phase flows through the support, alleviating many of the liquid handling issues associated with traditional LLE such as emulsion formation. As a result recoveries are often higher and demonstrate better reproducibility from sample to sample.

Now 25 years in business, Norlab is a specialist supplier of Separation Science & Dissolution Instruments and Accessories, Laboratory Fluid Handling Solutions and more...
To celebrate our 25th anniversary, we are planning special deals and promotions throughout the year. We encourage you to stay informed by signing up for our newsletter.

Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.

Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional Liquid Liquid Extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion formation and reduces sample preparation time by half.

Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis.

Converting a liquid-liquid extraction method for vitamin D to a Supported Liquid Extraction plate format
The objective of this study was to determine feasibility of transferring a validated Liquid-Liquid Extraction (LLE) method to a Supported Liquid Extraction (SLE) method using Biotage ISOLUTE SLE+ 96-well plates (400uL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples / not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.

47 drugs of abuse that can be extracted with Supported Liquid Extraction (SLE)
This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to UPLC-MS/MS analysis.


Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

Up to 20% off on Biotage ISOLUTE extraction columns and plates
We’re extending our 2016 Biotage ISOLUTE sample preparation products promotion into 2017. Price reductions are based on the quantity of a single order. However, your blanket order may contain multiple delivery and invoicing dates over a period of time. Up to four deliveries over a maximum time period of one year may be scheduled. Place an annual purchase order or a larger order and take advantage of our volume discounts without having to hold greater than necessary amounts of stock.

Biotage analytical sample preparation 2017
This catalog presents the extensive range of products for analytical sample preparation from Biotage.

Events & News

Now 25 years in business, Norlab is a specialist supplier of Separation Science & Dissolution Instruments and Accessories, Laboratory Fluid Handling Solutions and more...
To celebrate our 25th anniversary, we are planning special deals and promotions throughout the year. We encourage you to stay informed by signing up for our newsletter.

Articles

Up to 20% off on Biotage ISOLUTE extraction columns and plates
We’re extending our 2016 Biotage ISOLUTE sample preparation products promotion into 2017. Price reductions are based on the quantity of a single order. However, your blanket order may contain multiple delivery and invoicing dates over a period of time. Up to four deliveries over a maximum time period of one year may be scheduled. Place an annual purchase order or a larger order and take advantage of our volume discounts without having to hold greater than necessary amounts of stock.

Application notes


Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

Converting a liquid-Lliquid Extraction method for vitamin D to a Supported Liquid Extraction plate format
Date of creation: 
30 December 2015
Library code: 
10566
47 drugs of abuse that can be extracted with Supported Liquid Extraction (SLE)
Date of creation: 
13 January 2016
Library code: 
10582
Extraction of cocaine and metabolites from whole blood using ISOLUTE SLE+ prior to GC-MS analysis
Date of creation: 
14 January 2016
Library code: 
10583
Extraction of opiates from whole blood using ISOLUTE SLE+ prior to GC-MS analysis
Date of creation: 
14 January 2016
Library code: 
10585
Extraction of THC, Hydroxy-THC and Carboxy-THC from whole blood using ISOLUTE SLE+ prior to GC-MS analysis
Date of creation: 
14 January 2016
Library code: 
10587
Extraction of NBOMe designer drugs from oral fluid using ISOLUTE SLE+ prior to analysis by LDTD-MS/MS
Date of creation: 
14 January 2016
Library code: 
10588
Extraction of amphetamines and analogues from hair using ISOLUTE SLE+ prior to GC/MS analysis
Date of creation: 
27 September 2017
Library code: 
11288
Extraction of illicit drugs and pesticides from liver tissue using ISOLUTE SLE+ prior to GC/MS analysis
Date of creation: 
27 September 2017
Library code: 
11289
Acidic, basic and neutral drug screen of hydrolyzed urine using supported liquid extraction prior to LC-MS/MS analysis
Date of creation: 
28 September 2017
Library code: 
11291
Literature

Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.

Date of creation: 
30 December 2015
Library code: 
10563
Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate
Date of creation: 
30 December 2015
Library code: 
10565
Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
Date of creation: 
30 December 2015
Library code: 
10568
Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology
Date of creation: 
22 August 2017
Library code: 
11221
Date of creation: 
30 December 2015
Library code: 
10563
Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate
Date of creation: 
30 December 2015
Library code: 
10565
Biotage Extrahera Sample Preparation Automation System
Date of creation: 
3 December 2015
Library code: 
10482
Biotage Extrahera sample preparation automation system
Date of creation: 
24 August 2017
Library code: 
11231