Determination of optimal sample size with microelution without dry down using SPE for a drugs of abuse panel

Biotage Evolute Express sample preparation products provide robust, reliable SPE, giving ultra pure extracts and high analyte recoveries. Developed specifically for analyte quantification by LC-MS-MS, Evolute sorbents provide a highly effective solution to the problems of ion suppression and matrix effects.

In this note, an extraction protocol using a 10 mg mixed-mode cation exchange sorbent (Evolute Express CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving microelution off of the sorbent requiring no evaporation or reconstitution steps were also developed.