Norlab

Your partner in Northern Europe for innovative laboratory instruments

Viruses

Vertical Tabs

Overview

Characterization of viruses by Field-Flow Fractionation (FFF)
During the past decades, FFF has been applied successfully for the separation, characterization and fractionation of a wide variety of large-size materials, including viruses.

Quantification of adeno-associated virus aggregation using asymmetrical flow field-flow fractionation
In this application note, Postnova presents data on separation of AAVs and their aggregates using asymmetrical flow field-flow fractionation (AF4) and measurement of their radius of gyration (Rg or root mean square radius, R.M.S.) by multi angle light scattering (MALS).

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Postnova AF2000 MT mid temperature MultiFlow FFF system
The system can be configured for Asymmetric Flow Field-Flow Factionation (AF4), Hollow Fiber Flow Field-Flow Factionation (HF5) and Size Exclusion Chromatography (SEC).

Postnova CF2000 Centrifugal FFF system
The system can separate and distinguish relative size differences of 5% only and can be used to monitor absolute size differences down to 1 nm. These unique features make it the ideal choice for any nanoparticle research and QC tasks.

Postnova 21-angle MALS detector - the ideal detector for ultra-high molar mass
The Postnova 21-angle MALS detector has been especially developed for coupling with FFF systems – to the opposite of previously available MALS detectors which were designed with chromatography (SEC/GPC) in mind, that is for smaller molar mass.

High sensitivity online Dynamic Light Scattering detector, special customized for use connected to FFF
Online DLS measurements using a special flow cell can be perfomed to obtain particle size (Rh) and molecular weight (Mw) distributions of particles and molecules separated by Postnova Asymmetric Flow FFF (AF4), Centrifugal FFF (CF3) and Thermal FFF (TF3) systems.

Application notes

Quantification of adeno-associated virus aggregation using asymmetrical flow field-flow fractionation
In this application note, Postnova presents data on separation of AAVs and their aggregates using asymmetrical flow field-flow fractionation (AF4) and measurement of their radius of gyration (Rg or root mean square radius, R.M.S.) by multi angle light scattering (MALS).

Literature

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Date of creation: 
24 July 2017
Library code: 
10163
Date of creation: 
29 September 2015
Library code: 
10159
Date of creation: 
1 October 2015
Library code: 
10164
Multi-Angle Light Scattering detector (MALS) model 3621
Date of creation: 
27 July 2017
Library code: 
10220
Dynamic Light Scattering detector model Zetasizer Nano ZS173
Date of creation: 
1 October 2015
Library code: 
10221