Norlab

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Increased efficiency of the coomassie protein assay for protein content determination using simple automated liquid handling vs. manual procedures
In this application, the Gilson Pipetmax was used successfully to automate the 96-well coomassie protein assay, pipetting samples, standards, and reagents with comparable accuracy and consistency relative to manual results.

Gene expression signature in peripheral blood as a marker of parkinson’s disease
In this application by Gilson, five genes were selected based on the results of past global gene expression studies, and qPCR validation study was performed on samples taken from an independent subset of patients with PD and matched controls. The qPCR experiment was designed and prepared using the Pipetmax qPCR assistant software and the Pipetmax 268 automated pipetting instrument.

Gilson Pipetmax automation of the coomassie (bradford) protein assay using a multiple channel bench top
The bradford assay performed in this application was automated using the Pipetmax 268 bench top pipetting system. Absorbance of the coomassie dye bound to albumin protein was measured with the Vmax kinetic microplate reader. Results were compared with manual method performance using a combination of the Pipetman L single channel and Pipetman M multichannel pipettes.

Automating the Promega GoTaq PCR core system II reaction using the Gilson Pipetmax 268
In this application, the compact Pipetmax 268, a reproducible set-up, run and walk-away sample prep assistant, was used to prepare PCR reactions with the GoTaq PCR sore system II, and to compare its effectiveness versus a manually pipetted procedure.

Automated NGS library prep: ligation efficiency of NxSeq sample preparation using the Gilson Pipetmax 268
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAM-labeled adaptors using the NxSeq DNA sample preparation kit, comparing the manual method with an automated library prep protocol on the Pipetmax. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.

A flexible workflow for automated bioluminescent kinase selectivity profiling by Gilson
This note aims to show how ready-to-use kinase selectivity profiling systems (KSPS) combined with easy-to-use instrumentation and automated data analysis supports a streamlined workflow to enable quick and efficient in-house kinase profiling.

Nanoplastic analysis enabled by centrifugal field-flow fractionation coupled with multi angle light scattering and raman microscopy
The hyphenation of centrifugal field-flow fractionation (CF3) with UV, MALS and raman detection enables the simultaneous detection, sizing and chemical identification of polydisperse nanoplastic mixtures. Because of the wide range of polymers potentially present in nanoplastics, CF3 is an ideal separation technique as it can fractionate those particles with similar size but different mass or density.

Analysis of two API products using UV spectrophotometry with multi-component analysis and a fiber optic dissolution analyzer
This supplier article explains the theory behind the MCA algorithm methodology. Then, used in tandem with in-situ fiber optics, the accuracy of the technique is demonstrated by recovering the concentration of two APIs in known mixed solutions. Finally, an example is given of accurately monitoring and quantifying the dissolution profile of an actual commercial product containing two APIs, demonstrating the elimination of the need to draw samples or to perform HPLC analysis for many of these type of products.

Distek PrepEngine method development
This technical note goes through the method development process for the PrepEngine.

Analysis of two API products using UV spectrophotometry with multi-component analysis and a fiber optic dissolution analyzer
This poster explains the theory behind the MCA algorithm methodology. Then, used in tandem with in-situ fiber optics, the accuracy of the technique is demonstrated by recovering the concentration of two APIs in known mixed solutions. Finally, an example is given of accurately monitoring and quantifying the dissolution profile of an actual commercial product containing two APIs, demonstrating the elimination of the need to draw samples or to perform HPLC analysis for many of these type of products.

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